New insights in the interpretation of array-CGH: autism spectrum disorder and positive family history for intellectual disability predict the detection of pathogenic variants
© Cappuccio et al. 2016
Received: 20 October 2015
Accepted: 15 March 2016
Published: 12 April 2016
Array-CGH (aCGH) is presently used into routine clinical practice for diagnosis of patients with intellectual disability (ID), multiple congenital anomalies (MCA), and autism spectrum disorder (ASD). ACGH could detect small chromosomal imbalances, copy number variations (CNVs), and closely define their size and gene content. ACGH detects pathogenic imbalances in 14–20 % of patients with ID. The aims of this study were: to establish clinical clues potentially associated with pathogenic CNVs and to identify cytogenetic indicators to predict the pathogenicity of the variants of uncertain significance (VOUS) in a large cohort of paediatric patients.
We enrolled 214 patients referred for either: ID, and/or ASD and/or MCA to genetic services at the Federico II University of Naples, Department of Translational Medicine. For each patient we collected clinical and imaging data. All the patients were tested with aCGH or as first-tier test or as part of a wider diagnostic work-up.
Pathologic data were detected in 65 individuals (30 %) and 46 CNVs revealed a known syndrome. The pathological CNVs were usually deletions showing the highest gene-dosage content. The positive family history for ID/ASD/MCA and ASD were good indicators for detecting pathological chromosomal rearrangements. Other clinical features as eyes anomalies, hearing loss, neurological signs, cutaneous dyscromia and endocrinological problems seem to be potential predictors of pathological CNVs. Among patients carrying VOUS we analyzed genetic features including CNVs size, presence of deletion or duplication, genic density, multiple CNVs, to clinical features. Higher gene density was found in patients affected by ID. This result suggest that higher gene content has more chances to include pathogenic gene involved and causing ID in these patients.
Our study suggest the use of aCGH as first-tier test in patients with neurdevelopmental phenotypes. The inferred results have been used for building a flow-chart to be applied for children with ID.
KeywordsIntellectual disability aCGH Copy number variant (CNV) Pathogenic CNV Multiple congenital anomalies
ID (intellectual disability) and ASD (autism spectrum disorder) are life-long conditions with deficits in cognitive functioning (IQ<70) and adaptive skills that affects 1–3 % of children worldwide . Array-CGH (aCGH) offers a high diagnostic yield, ranging from 14–20 %, for individuals with unexplained ID, ASD or multiple congenital anomalies (MCA) [2–5]. Available evidence suggests a change in the diagnostic approach for children with neuropsychiatric disorders and/or congenital anomalies, indicating the aCGH as the first-tier cytogenetic diagnostic test . In 2014 the SIGU (Italian Society of Human Genetics) suggested that ID, of all severity, and/or ASD and/or epilepsy, hypotonia, dysmorphisms, growth alteration, congenital malformations might be associated with pathogenic aCGH results.
The chromosomal imbalances detected by aCGH are defined copy number variations (CNVs) that are referred as: microdeletions and microduplications of clear clinical relevance or pathogenic, variants of uncertain significance (VOUS) and benign polymorphisms [6–8]. The advances in molecular methodology of aCGH technology, along with its broader application, facilitated the detection of novel pathogenic CNVs. The significance of many VOUS still remains uncertain causing serious problems in defining their contribution in patients affected by ID, MCA and ASD [9–13].
To determine phenotypic clues associated to pathogenic CNVs, outlining criteria for selecting patients to be studied with aCGH as first-line test.
To identify cytogenetic criteria of VOUS to be taken into account for their potentially pathogenicity.
These aims allow us to depict an integrative flow-chart applying for children with ID.
We present a retrospective-prospective study including 214 patients. In order to establish which patients could mostly benefit of aCGH as first molecular test, we tried to individuate clinical and anamnestic clues that significantly correlate with pathologic aCGH data. For this purpose, all the subjects received a complete history recall and an accurate clinical and instrumental evaluation. All the anamnestic-clinical-investigation data, either as a single feature or in combination, were correlated to pathologic or negative aCGH results.
Our second aim was to identify cytogenetic elements that could predict the pathological role of VOUS. We characterized meticulously all the VOUS detected, recording: the size of the rearrangements, the presence of deletions, duplications or multiple rearrangements and the genic density. We correlated the cytogenetic data with the severity of the phenotype. A severe phenotype was defined on the presence of severe ID, ASD and multiple malformations.
On the basis of inferred results, we define a flow-chart applying for children with ID.
Patients and methods
We enrolled 214 patients (114 males and 100 females with an average age of 5.63 years, range 1.3–19 years), with or without variable degree of ID, and/or ASD and/or MCA. They had been referred and evaluated at the Clinical Genetic Unit of the Translational Medical Science Department, section of Pediatrics, of the University of Naples “Federico II” in a 10-year period (2002–2012): 91 patients were recruited (47 male and 44 female, age range 1.7–19 years, mean 5.1 years) from 2002–2006, and followed up, so belonged to a retrospective part of the study; 123 patients (69 male and 54 female, age range 8 month-11years, mean 6.16 years) were enrolled in the prospective part of the study from 2006–2012.
The diagnostic workup started with a meticulous family history recall, physical examination focused on the presence of minor (dysmorphology evaluation) and major anomalies, neurological exam and assessment of the behavioral phenotype as detailed in the Additional file 1 .
The diagnostic instrumental evaluation included: brain MRI, abdominal ultrasound, echocardiography, EEG and ABR. Not all the investigations were performed due to the lack of patient cooperation or parental consent. For patients who underwent brain MRI the association between brain structural anomalies and the presence of ID (moderate/severe), microcephaly, macrocephaly, hearing loss, EEG abnormalities was evaluated.
Metabolic screening tests
A metabolic screening panel (plasma amino acids, acylcarnitine profile, urine organic acids, ammonia and lactic acid) was performed. Targeted metabolic tests (lysosomal enzymes, urine oligosaccharides, transferrin isoform profile, plasma sterol concentrations, congenital glycosylation defects) were carried out when clinical suspect arose.
For patients included in the retrospective part, initial tests to exclude genetic syndromes suspected based on the facial and gestalt phenotypes or due to initial unavailability of aCGH were performed, including: karyotype (standard and high resolution type), specific loci (22q11.2, 7q11.23, 16p13.3) and/or subtelomeric FISH, DNA methylation analysis for Prader-Willi and Angelmann syndrome and FRAX-A/E test. Since a diagnosis was not achieved through these procedures, aCGH with an average resolution of 500 Kb was performed. In patients enrolled in the prospective part of the study, aCGH with a resolution of 50–75 Kb was used as first tier test. Different platforms have been used along with progressive improvement of aCGH technology. Despite this could represent a bias we could not analyze again all the patients previously studied in the retrospective part with a lower resolution power. We re-analyzed only few patients with clinical features strongly suggestive for chromosomal disorder and showing normal results. These results have been considered in the prospective part of the study.
Cytogenetic techniques applied and CNVs interpretative processes are included in Additional file 1 .
The CNVs size, the presence of multiple rearrangements and the number of genes located in were assessed. Patients with overlapping chromosomal rearrangements have been compared and studied.
Clinical data were assessed using a standardized protocol (excel sheet) for collection of data in all children. SPSS software (version 20.0; SPSS Inc., Chicago, IL, USA) and R (version 2.5.0; The R Foundation for Statistical Computing) were used for the statistical analysis. Analyses included only available data and missing values were not imputed. Data were summarized as means ± standard deviation (median [25th–75th percentile]) for continuous variables and as frequencies (%) for categorical variables. The association between the presence of a pathogenic CNV (as opposed to a normal array CGH results) with clinical characteristics (including ID, ASD, and familiarity with ID and with several anomalies/malformations) was based on a standard two-step approach. In particular, univariate association between each predictor and the aCGH results were first established using chi-square test (or the fisher exact test when appropriate); afterward, those factors showing a bivariate association with the dependent variable at a p<0.2 were entered in a multivariate logistic regression model using a backward stepwise method for the selection of variables. The discriminatory ability of the final model was assessed using ROC curve analysis. In order to correct the area under the ROC curve (AUC) for over-optimism, which occurs when the fit of a model is evaluated using the same data in which the model was built, a bootstrap procedure as described in  was used. The exact Mann–Whitney U test was used for comparing children with and without ID in case of VOUS with respect to the following variables: presence of deletions/duplications, number of multiple rearrangements, average size of rearrangements and genetic density.
Clinical features recorded in each patient expressed as percentage in are provided
Percentage of patients
Autism spectrum disorder
Familiarity for ID and/or MCA
Prenatal perinatal problems
Microcephaly (Craniosynostosis included)
Forehead and eyebrows phenotypic abnormalities
Eyes, palpebral fissures and eyelashes phenotypic abnormalities
Nose and phyltrum phenotypic abnormalities
Oral region, teeth and tongue phenotypic abnormalities
Ears phenotypic abnormalities
Neck and thorax phenotypic abnormalities
Upper limbs, lower limbs, hands and feet phenotypic abnormalies
Gastrointestinal malformations (megacolon, Duodenal/Esophageal stenosis)
Neurology (Epilepsy, hypertonia, hypotonia, paresis, extrapyramidal signs)
Kidney and urinary tract anomalies
Abnormal external genitalia
Nails and hair anomalies
Alterated skin pigmentation or skin hemangioma
Genetic and Instrumental tests performed in the cohort are indicated
Percentage of patients testing pathologic
Percentage of patients performing the test
Subtelomeric rearrangements analysis
Other molecular analyses
Plasma amino acids and acylcarnitines
Urine organic acids
Plasmatic ammonia and lactic acid
Serum transferrin isoform profiling
The metabolic screening panel consisting in: plasma amino acids, acylcarnitine profile, urine organic acids, ammonia and lactic acid were applied in around 50 % of patients. In specific cases lysosomal storage disease were partially ruled out performing lysosomal enzymes assay, urine oligosaccharides and glycosaminoglycans. Transferrin isoform profiling and sterols were performed only in 9 and 6 % of patients (Table 2).
Cytogenetic analysis results
Cytogenetic features of detected CNVs
Average size of chromosomal rearrangement (kb)
Multiple chromosomal rearrangements (number of patients)
Pathological CNV (65)
The diagnosis of already known genetic syndromes was achieved in 47 patients (data not shown). For Cri-du-chat syndrome, DiGeorge syndrome, 1q21.1 deletion syndrome, 1q21.2 deletion syndrome, 3q29 deletion syndrome, 6p25 deletion syndrome, 6q duplication syndrome, 13q12.3 deletion syndrome and 16p11.2 deletion syndrome, more than one case was recorded. The comparison of these cases might widen the phenotype associated to microdeletion syndromes and might contribute to a more careful study of critical regions containing haplosensitive genes. Furthermore, 18 pathogenic novel chromosomal rearrangements have been discovered as well.
Correlation study results
Correlations between single and combined clinical features and pathogenic and negative aCGH results are shown
Clinical signs, dysmorphic features
aCGH results (number)
Autism Spectrum Disorders
Familiarity for ID/MCA/ASD
Prenatal perinatal problems
Microcephaly (or craniosynostosis)
Forehead and Eyebrows dysmorphisms
Eyes, palpebral fissures and eyelashes dysmorphisms
Nose and philtrum dysmorphisms
Oral region, teeth and tongue dysmorphisms
Neck and thorax anomalies
Upper and lower limbs, hands, feet dysmorphisms
Neurologic signs (Epilepsy, hypertonia, hypotonia, paresis)
Kidney and urinary tract anomalies
Abnormal external genitalia
Nails and hair anomalies
Alterated skin pigmentation
Characterization of uncertain CNV (VOUS)
Evaluation of cytogenetic indicator in VOUS suggests that gene density is the only parameter associated to ID
Deletion; n (%)
3 (75 %)
10 (47.6 %)
13 (52 %)
CNV dimension; Median [25th 75th percentile]
Genic densitiy; Median [25th 75th percentile]
Multiple CNVs; Median [25th 75th percentile]
Suggested diagnostic flow-chart
ID is a developmental disability, which presents in infancy or early childhood characterized by impaired intellectual functioning and adaptive behavior. The prevalence is estimated between 1 and 3 % of children [5, 20–22]. ASDs are clinically heterogeneous disorders that include: autism, Asperger syndrome, pervasive developmental disorders not otherwise specified, and childhood disintegrative disorder. ASD has been estimated to affect 1/100 to 1/150 children . Many children with ASD also have ID representing together the most frequent referral to geneticists for a diagnostic workup. American Academy of Pediatrics Committee has discussed the importance of early identification of the cause of neurocognitive phenotype identifying several benefits . Since even small chromosomal anomalies have been established as a major cause of ID and ASD, aCGH test has become an important diagnostic tool for patients at least sharing neuro-phenotypes [2–5].
Despite a very high number of studies describing genetic findings of CNVs, we identifed a high rate of pathologic CNVs, more frequently deletions, in a cohort of patients with ID and/or ASD. High gene content is also found in patients with VOUS and ID.
We also demonstrated that a positive family history for ID/ASD/MCA (cardiologic, renal, intestinal anomalies) and ASD were two good independent indicator of pathologic CNVs.
On the basis of our data we suggest that aCGH should be used as first tier diagnostic test in the presence of ID/ASD/MCA. We also underline the importance of family historical recall and of parents’ clinical observation in order to evaluate the presence of ID/ASD in the parents.
Among VOUS, higher gene density was found in patients affected by ID.
A diagnosis of metabolic disorders has been reported in 1–5 % of patients with ID [3, 19, 23, 24]. Diagnostic evaluation for inborn errors of metabolism (IEMs) was performed in 126 patients giving normal data. Notwithstanding, metabolic studies should be thoroughly considered in patients with ID due to the potential treatability of IEMs.
ACGH was performed in patients included in the retrospective part of this study because of negative results of other genetic studies, while it was applied as first tier test in the prospective part of the study because growing number of papers highlighted its diagnostic power [25–27]. We identified 65 (30 %) and 25 (12 %) patients with pathogenic CNVs and VOUS respectively. The detection rate of clinically significant CNVs is about 30 % therefore higher than the yields (from 14.2–21.1 %) obtained from studies that used similar platforms [2, 3, 5, 10, 28–30]. We speculate that the higher prevalence of pathological CNVs in our study is potentially ascribed to the careful selection of patients. Among patients with pathological CNVs, the deletions were more frequent than duplications (65 vs 42) being the former more commonly interpreted as pathogenic . The pathogenic CNVs have a higher size (7 Mb) and gene density (35 genes) than VOUS and benign ones in agreement with other reports [29, 31, 32]. 29 patients with pathological CNVs have multiple rearrangements, which are known to exacerbate neuro-developmental phenotypes (Table 3) [5, 32]. In these patients parental chromosomal study did not reveal balanced translocations.
We diagnosed 47 patients (22 %) with OMIM syndromes on the basis of either overlapping described well- microdeletion/microduplication syndromes or known causing-genes mapped within chromosomal rearrangements. ACGH detected CNVs scattered throughout the genome, but the chromosome 1, 8, 22, X resulted most frequently involved in line with other reports . The non-random involvement of specific chromosomal segments could be the results of non-allelic homologous recombinant mutational mechanism . Other recurrent pathogenic CNVs involved 1q21.1, 1q41q42, 2p15, 16p13.1, 16p11.2, 17q21.31 allowing us to characterize the phenotypes associated to chromosomal rearrangements in these specific regions [30, 34–39]. A specific chromosomal abnormality does not always correspond to a specific or suggestive phenotype. In such cases, the detection of genomic aberration precedes the definition of specific phenotypes . In our dataset patients with 22q11.21 deletion and Cri-du-chat syndromes showed an atypical phenotype making the clinical diagnosis challenging .
The potential limitations of aCGH application regard: delayed turnaround time, the impossibility of the detection of balanced translocations and low-level mosaicisms, the high costs, so that clinical criteria for selection of patients with higher probability of pathogenic CNV are desirable.
The selection of patients who are most likely to have a diagnosis by aCGH, minimizing the number of benign CNVs or negative results, remains an attractive goal . This study represents the largest collection of specific clinical and instrumental data for which an association with pathologic CNVs has been investigated. From previous studies, the same rate of pathologic chromosomal imbalances by aCGH was found in unselected and selected patients ([18, 41] respectively). Other studies found the higher frequency of pathogenic CNVs in patients with congenital anomalies, unspecified dysmorphisms, growth anomalies, heart defects, primary microcephaly and familial occurrence of ID [22, 42–44]. The diagnostic yield among patients with more severe ID would be expected to be higher than in patients with milder ID [45–47]. In our cohort, 47 patients were enrolled because of MCA and they did not show ID. Indeed pooling data from patients with different ID degree and without ID, we conclude that more severe ID is not statistically related to pathogenic CNVs [4, 5, 29]. Among the consistent number of clinical and history data analyzed, positive family history for ID/MCA/ASD and isolated ASD were found to be associated to pathological aCGH results. We would underline that other congenital anomalies as ocular dysmorphisms (p=0.062), hearing loss (p=0.127), neurological signs (p=0.103), cutaneous dyscromia (p=0.08) and endocrinological system involvement (p=0.128) are potentially predictors of pathological CNVs.
In this cohort, 37 patients were affected by ASD. The overall diagnostic yield of aCGH for patients with ASD ranges from 18.2–22 % [45, 48–50]. In our cohort the diagnostic yield is consistently greater (around 44 %, 16 patients out 37). Among ASD patients of this case-study, pathogenic CNVs are mostly located at chromosome 1, 4, 6, 8, 21 and 22, that partially confirm the previous results from the literature [45, 51]. In the present study we found a low frequency of abnormal FRAX-A test results as previously described .
Some hesitations in using aCGH in clinical setting diagnostic test derive from the difficulties in the efficient discrimination between benign, VOUS and pathogenic CNVs [2, 11, 52]. CNVs can be interpreted as abnormal (pathological CNVs), VOUS and benign. We interpreted CNVs as pathogenic when contained: critical regions of microdeletion/microduplication known syndromes, genes associated with autosomal dominant inherited diseases and when cases with similar phenotypes and overlapping CNVs have already reported. CNVs are likely to be benign if they are reported in controls databases (similar CNVs in at least three healthy individuals in the same “sense”, with an overlap of more than the 50 % and the not-overlapped part less than 100 Kb), if they do not contain genes and/or known regulatory elements. Comparative analysis, with data listed in available large datasets, guide toward the clarification of CNVs clinical impact and interpretation. Multiple sources were considered as level of documentation. All the identified CNVs have been compared to those listed in: the Database of Genomic Variants (DGV, http://projects.tcag.ca/variation) that includes healthy individuals, the pathogenic CNVs databases for patients with ID, ASD and MCA: as the International Standard Cytogenomic Array Consortium Databases (ISCA, https://www.clinicalgenome.or), as well as the Database of Chromosomal Imbalance and Phenotype in Humans using Ensemble Resources (DECIPHER, https://decipher.sanger.ac.uk/). The Database of Genomic Structural Variation (dbVar, http://www.ncbi.nlm.nih.gov/dbvar) including structural variation from both normal control population and disease population has been consulted as well. The genes, involved in the chromosomal region of interest, and their functions have been checked by UCSC Genome Browser (http:// http://genome-euro.ucsc.edu/cgi-bin/hgGateway) and Ensamble Genome Browser (http://www.ensembl.org/index.html). In the interpretative process, each gene, within the CNV as well as neighboring genes, was studied for its potential role in neurological development, by all the available evidence along sources as OMIM, Genereviews, PubMed. The CNVs not associated with previously reported pathogenicity or benignity criteria were estimated as VOUS. The potential pathogenicity of VOUS is reported to be determined by many factors: the “sense” of the rearrangement (deletion or duplication, as the penetrance of duplications is considered lower than of deletions), the size (pathogenic imbalances tend to be larger than benign) and the gene content . We detected and analyzed 25 de novo VOUS and evaluated some cytogenetic indicators: overall size, gain vs loss, presence of multiple rearrangements (complex rearrangements involving several CNVs) and gene content. Only gene content had a significant correlation with ID. The gene content should be evaluated in order to speculate the pathogenicity of VOUS [7, 10, 53].
Due to presence of VOUS, incomplete penetrance, and variable expressivity of CNVs the role of genetic counseling in aCGH testing and CNVs interpretation complements the diagnostic testing. Moreover pre-test counseling cannot be underestimated and should review potential benefits and limitations of the test.
The achievement of a specific genetic diagnosis improves medical care and allows an accurate recurrent risk counselling for the family. ACGH enables: discovering emerging new syndromes and variable presentations of already characterized ones, deciphering the genetic bases of many syndromes by discovering candidate genes. The current study highlights that a checklist of clinical features for preselecting cases for aCGH analysis with high sensitivity and specificity is difficult to attain. The positive family history for ID/MCA/ASD and the presence of ASD seem to be independent additional clues positively associated to causative CNVs. Among other criteria, ID (with no correlation with the level), eyes anomalies, hearing loss and other neurological signs, cutaneous dyscromia, endocrinological system involvement were also deemed as potentially predictive factor of pathogenic CNVs. VOUS involving gene-rich regions are more frequently associated to ID and pathological phenotypes.
What’s Known on This Subject
Array-CGH has been defined the first line diagnostic tool in patients sharing intellectual disability and multiple congenital abnormalities. The diagnostic yield is still low and interpretative issues of the results remain elusive.
What This Study Adds
Phenotypic clues predictive of pathological results have been defined to help in patients’ selection to be studied with array-CGH. Cytogenetic features of rearrangements to be taken into account are provided; variant of uncertain significance showed higher density in patients sharing intellectula disability. A revised flow-chart for patients with intellectual disability is depicted.
This retrospective-prospective study was approved by the Ethics Committee of Federico II University (reference number 5416), and was performed according to the Italian regulations on privacy protection and Helsinki Doctrine for Human Experimentation.
brainstem auditory evoked potentials
autism spectrum disorder
copy number variation
inborn errors of metabolism
multiple congenital anomalies
Magnetic Resonance Imaging
variant of uncertain clinical significance
This work was partially supported by a Grant from A multicenter collaborative research network for the identification and study of rare undiagnosed patients: the impact on the rare disease National Health Service network (UnRareNet)" - RF-2010-2312766" to G.A.
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