ICAM 1 expression in BEAS-2B cells. Flow cytometric analysis of ICAM-1 expression on cells: A) untreated (CTR), treated with TNF-α or pidotimod (pid 10 and 100 μg/ml) for 24 h; B) treated with 100 μg/ml pidotimod for different time periods (24, 36 and 48 h); C) pre-treated with pidotimod for different time (4, 12 and 24 h) and then stimulated with TNF-α for additional 24 h. ICAM-1 expression is quantified as mean fluorescence intensity (MFI) on the ordinate and the different culture conditions are shown on the abscissa. The data are expressed as mean ± SEM. *p < 0.05 versus control. The results shown are representative of three independent experiments.