Immunofluorescence and flow cytometry analysis of TLR-2 expression induced by pidotimod. (A) Representative images of TLR-2 expression collected by fluorescence microscopy in cell cultures untreated (CTR) or treated with TNF-α, zymosan, pidotimod (Pid 100 μg/ml) or pidotimod (Pid 100 μg/ml) plus TNF-α for 24 h; B) Histograms displaying the flow cytometric analysis of TLR-2 expression on cells treated with TNF-α, zymosan, pidotimod (Pid 10, 100 μg/ml), or pidotimod (Pid 100 μg/ml) plus TNF-α (TNF-α Pid or zymosan (Zym + Pid, for 24h. TLR-2 expression is quantified as mean fluorescence intensity (MFI) on the ordinate and the different culture conditions are shown on the abscissa. The data are expressed as mean ± SEM.*p < 0.05 versus control. The results shown are representative of three independent experiments.