Western blot analysis of TLR-2 expression induced by pidotimod. (A) Representative western blot of TLR-2 and GAPDH expression of BEAS-2B incubated with medium alone (CTR) or treated with TNF-α, zymosan (Zym), pidotimod (Pid 100 μg/ml) or pidotimod (Pid 100 μg/ml) plus TNF-α or zymosan (Zym), for 24 h.(B) The densitometric analysis of TLR-2 expression, normalized to GAPDH, is shown on the ordinate and the different culture conditions on the abscissa. The data are expressed as mean ± SEM. *p < 0.05 and **p < 0.01 versus control. The results shown are representative of three independent experiments.