Detection by Western blot of ERK1/2 phosphorylation. (A) Representative western blot of phosphorylated and total ERK1/2 of BEAS-2B cells untreated (CTR) or exposed to TNF-α or pidotimod (Pid) for 5 min or TNF-α with pidotimod (TNF-α + Pid) for 5 to 60 min. (B) The densitometric analysis of ERK1/2 phosphorylation, normalized to total ERK1/2 is shown on the ordinate and the different culture conditions on the abscissa. The data are expressed as mean ± SEM.*p < 0.05 versus control. The results shown are representative of three independent experiments.