Detection by Western blot of NF-kB expression. (A) Representative western blots for NF-kB p65 levels in the cytoplasm and in the nuclear extracts of BEAS-2B cultured with medium alone (CTR) or exposed to TNF-α, 100 μg/ml pidotimod (Pid 100), or TNF-α and pidotimod (TNF-α + Pid 100) for 24 h. GAPDH and Laminin A/C were used as housekeeping protein. (B) The densitometric analysis of NF-kB data, normalized to GAPDH (cytoplasm) or laminin A/C (nucleus), are shown on the ordinate and the different culture conditions on the abscissa. The data are expressed as mean ± SEM. *p < 0.05 versus control. The results shown are representative of three independent experiments.