Table 4 List of proteins identified from bands showing changes after Holder pasteurization
From: Effects of Holder pasteurization on the protein profile of human milk
Band no. | Protein ID | AC number (gi NCBI) | Theoretical Mr (kDa) | MASCOT score | Sequence coverage (%) | MS/MS sequencing |
|---|---|---|---|---|---|---|
1 | TNC variant protein | 68533131 | 244.2 | 1177 | 15 % | R.LEELENLVSSLR.E |
K.FTTDLDSPR.D | ||||||
R.ELEPGVEYFIR.V | ||||||
R.VFAILENKK.S | ||||||
R.VATYLPAPEGLK.F | ||||||
R.QTGLAPGQEYEISLHIVK.N | ||||||
R.LDAPSQIEVK.D | ||||||
K.ETFTTGLDAPR.N | ||||||
R.VSQTDNSITLEWR.N | ||||||
K.TTLTGLRPGTEYGIGVSAVK.E | ||||||
K.EDKESNPATINAATELDTPK.D | ||||||
K.ESNPATINAATELDTPK.D | ||||||
R.GLEPGQEYNVLLTAEK.G | ||||||
K.AATPYTVSIYGVIQGYR.T | ||||||
R.AVDIPGLEAATPYR.V | ||||||
R.TPVLSAEASTAK.E | ||||||
K.QSEPLEITLLAPER.T | ||||||
R.EATEYEIELYGISK.G | ||||||
R.APTAQVESFR.I | ||||||
K.FTTDLDSPR.D | ||||||
R.DLTATEVQSETALLTWRPPR.A | ||||||
K.EVIVGPDTTSYSLADLSPSTHYTAK.I | ||||||
K.IQALNGPLR.S | ||||||
R.EEFWLGLDNLNK.I | ||||||
K.ITAQGQYELR.V | ||||||
R.DHGETAFAVYDKFSVGDAK.T | ||||||
2 | Beta-casein | 288098 | 25.2 | 63 | 12 % | K.SPTIPFFDPQIPK.L |
K.VLPIPQQVVPYPQR.A | ||||||
3 | Beta-casein | 288098 | 25.2 | 65 | 12 % | K.SPTIPFFDPQIPK.L |
K.VLPIPQQVVPYPQR.A | ||||||
4 | Lactotransferrin | 16198359 | 78.3 | 333 | 8 % | R.YYGYTGAFR.C |
R.THYYAVAVVK.K | ||||||
R.SDTSLTWNSVK.G | ||||||
R.CLAENAGDVAFVK.D | ||||||
R.RSDTSLTWNSVK.G | ||||||
K.LRPVAAEVYGTER.Q | ||||||
Immunoglobulin kappa light chain VLJ region | 21669409 | 29.8 | 118 | 17 % | R.TVAAPSVFIFPPSDEQLK.S | |
K.DSTYSLSSTLTLSK.A | ||||||
K.VYACEVTHQGLSSPVTK.S | ||||||
Beta-casein | 288098 | 25.2 | 106 | 28 % | K.SPTIPFFDPQIPK.L | |
K.VLPIPQQVVPYPQR.A | ||||||
R.AVPVQALLLNQELLLNPTHQIYPVTQPLAPVHNPISV.- | ||||||
Immunoglobulin lambda light chain | 33700 | 25.2 | 77 | 20 % | R.SYSCQVTHEGSTVEK.T | |
K.YAASSYLSLTPEQWK.S | ||||||
K.AAPSVTLFPPSSEELQANK.A | ||||||
5 | Immunoglobulin kappa light chain VLJ region | 21669357 | 29.5 | 381 | 34 % | K.LLIYWASTR.E |
K.DSTYSLSSTLTLSK.A | ||||||
K.SGTASVVCLLNNFYPR.E | ||||||
K.LYACEVTHQGLSSPVTK.S | ||||||
R.TVAAPSVFIFPPSDEQLK.S | ||||||
K.VDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSK.A | ||||||
Alpha S1-casein | 1359714 | 20.7 | 192 | 19 % | R.LQNPSESSEPIPLESR.E | |
R.LNEYNQLQLQAAHAQEQIR.R | ||||||
Immunoglobulin lambda light chain | 33700 | 25.2 | 139 | 14 % | K.YAASSYLSLTPEQWK.S | |
K.AAPSVTLFPPSSEELQANK.A | ||||||
Beta-casein precursor | 4503087 | 25.4 | 115 | 11 % | K.SPTIPFFDPQIPK.L | |
K.VLPIPQQVVPYPQR.A | ||||||
6 | Beta-casein | 288098 | 25.2 | 49 | 12 % | K.SPTIPFFDPQIPK.L |
K.VLPIPQQVVPYPQR.A | ||||||
7 | Alpha S1-casein | 1359714 | 20.7 | 56 | 10 % | R.LNEYNQLQLQAAHAQEQIR.R |
Beta-casein | 288098 | 25.2 | 50 | 12 % | K.SPTIPFFDPQIPK.L | |
K.VLPIPQQVVPYPQR.A | ||||||
8 | Beta-casein | 288098 | 25.2 | 38 | 12 % | K.SPTIPFFDPQIPK.L |
K.VLPIPQQVVPYPQR.A | ||||||
9 | Beta-casein | 288098 | 25.2 | 81 | 12 % | K.SPTIPFFDPQIPK.L |
K.VLPIPQQVVPYPQR.A | ||||||
tenascin | 37227 | 240.6 | 70 | 1 % | R.LEELENLVSSLR.E | |
alpha S1-casein | 1359714 | 20.7 | 65 | 10 % | R.LNEYNQLQLQAAHAQEQIR.R | |
10 | Lactoferrin | 186833 | 78.3 | 203 | 3 % | R.THYYAVAVVK.K |
K.LADFALLCLDGK.R | ||||||
K.NLLFNDNTECLAR.L | ||||||
Beta-casein | 288098 | 25.2 | 38 | 6 % | K.VLPIPQQVVPYPQR.A |