Fig. 4
From: The role of extracellular histones in systemic-onset juvenile idiopathic arthritis
Serum histone induces neutrophils to release NETs,heparin reduces histone -induced NET production. A: 0 μg/ml histone, B. 10 μg/ml histone, C. 20 μg/ml histone, D. 50 μg/ml histone, E. 0 μg/ml histone, F. 10 μg/ml histone+heparin, G. 20 μg/ml histone+heparin, H. 50 μg/ml histone+heparin, incubate with neutrophils for 4 h; I. the proportion of neutrophils that produced NETs, and I. the MPO levels in the supernatant when neutrophils incubate with 0 μg/ml, 10 μg/ml, 20 μg/ml and 50 μg/ml histone +heparin; K. the proportions of diffused and spread neutrophils in the 10 μg/ml and 20 μg/ml histone . *** p < 0.001;** P < 0.01;* p < 0.05;n.s p > 0.05