Congenital nephrotic syndrome associated with 22q11.2 duplication syndrome in a Chinese family and functional analysis of the intronic NPHS1 c. 3286 + 5G > A mutation

Table 1 primers used in minigene assay

Primers
F	TGTTGCCTAGGCTGGTCTTG
R	GTCCTCTTCCGACCTTCCAG
F′	TTGTGGAGATGGGGGTGGAGATGG
R’	ACACATGGCTTTAGGCTTTGATCCC
SD6	TCTGAGTCACCTGGACAACC
SA2	ATCTCAGTGGTATTTGTCAGC

F and R were used to amplify the genomic fragments of exon 24, intron 24 and exon 25 of NPHS1 from the proband and healthy control. F′ and R’ were used to amplify the vector pSPL3, SD6 and SA2 were used to amplify and sequence the RT-PCR products from COS7 cells

ISSN: 1824-7288