Fig. 1

The lungs of TLR2^−/− mice showed less inflammation following OVA sensitization compared with wild-type mice. A Lungs were fixed, embedded in paraffin and cut into 3-μm thick sections. Lung sections were stained with hematoxylin and eosin (HE) and photographed using an Olympus microscope. Magnification: 200-fold; B Cytokines were measured in Lung tissue or B-cell by RT-PCR. Data are shown individually and as the mean ± s.e.m. IL4, IL13 and IL21 expression decreased more significantly following OVA sensitization in TLR2^−/− mice compared with wild-type mice as shown. Data for IL4, IL5, IL13 and IL21 were normalized to wild-type mice treated with normal saline (NS). *: p < 0.05, n = 6 mice per group