Fig. 3
From: The role of extracellular histones in systemic-onset juvenile idiopathic arthritis
Free ferric ions induce neutrophils to produce NETs, and heparin reduces free iron-induced NET production. A: 0 μg/ml FeCl3, B. 1 μg/ml FeCl3, C. 2 μg/ml FeCl3, D. 5 μg/ml FeCl3, E. 0 μg/ml FeCl3 + heparin, F. 1 μg/ml FeCl3 + heprin, G. 2 μg/ml FeCl3 + heparin, H. 5 μg/ml FeCl3 + heprin incubate with neutrophils for 4 h; I. the proportion of neutrophils that produced NETs, and J. the supernatant MPO levels when 0 μg/ml、1 μg/ml、2μg/ml and 5 μg/ml FeCl3 + heparin incubate with neutrophils for 4 h. *** p < 0.001;** P < 0.01;* p < 0.05;n.s p > 0.05e