Table 1 Primer sequences, PCR product size, restriction enzymes and band sizes of SNPs selected for this study
SNPs | Gene | Primer sequence | PCR product size | Restriction Enzyme | Restriction Fragment Size |
|---|---|---|---|---|---|
rs1017 NG_023040.1:g.16138A > T | ISL1 | F-CTCTTGGCCTGTCCTGTAGC R-GCAATGCAAGAGCAAACAAA | 318 bp | DraI | AT: 201, 117,95 and 22 bp AA: 201 and 117 bp TT:201, 95 and 22 bp |
NC_000006.10:g.126117350A > C Asp98Ala (293A > C) | HEY2 | F-GCCACAAGTACCCAGAAGAAAC R-GCACAAGTCTTCTCAACTCAG | 340 bp | MboI | 296 bp 44 bp |
rs36208048 NG_008732.1:g.3877C > A | VEGF | F-AACCCCCATTTCTATTCAG R-CTGTGGAGTCTGGCAAAA | 278 bp | AlwN1 | Wild type cut at C 146 bp 132 bp |
rs7240256 NG_029226.1:g.23449 T > C | NFATc1 | FI- GGTCACATGCAGCAGCGCT RI- TGACATTTTCCACGCCTGACG FO-GGTTTGCAGTTAACCTTTTCCCA RO-AGAAAGCTCCTTCTGGCATAGG | ––– | ––– | 196 bp (T allele wild type) 239 bp (C allele SNP) 384 bp outer primers |
rs11067075 NG_007373.1:g.51682G > T | TBX5 | FI- GTGATAAGGAATCAGCCGGGT RI- GAATCCCAACTGGAAGGAGAC FO- TCCTGCCTAGGAGACAACAAATA RO-AGACAATGAGGGGAAGTCAGATA | ––– | ––– | 124 bp T allele 99 bp (wild type, G allele) 171 bp outer primers |
rs1801133 NG_013351.1:g.14783C > T | MTHFR | OF-GCTGTTGGAAGGTGCAAGATCA OR-GAGTGGGGTGGAGGGAGCTTAT IF-AGAAGGTGTCTGCGGG IR-AAAGCTGCGTGATGAAAT | ––– | ––– | 177 bp for T allele 230 for C allele 366 bp outer primer |