- Open Access
Nutritional status of adolescents with cystic fibrosis treated at a reference center in the southeast region of Brazil
© Del Ciampo et al. 2015
- Received: 6 January 2015
- Accepted: 15 July 2015
- Published: 30 July 2015
Several factors can interfere with the full physical and emotional growth of adolescents, among them chronic diseases. The aim was to determine the nutritional status of adolescents and to associate it with puberty, pancreatic sufficiency, lung function and age range of Cystic Fibrosis (CF) diagnosis.
An observational, cross-sectional, retrospective and analytical study was conducted using the data of medical records. Setting: Reference center in the northeastern region of the state of São Paulo – Brazil. Patients: All adolescents with CF attended in 2010 were included. Some variables included: pancreatic sufficiency (steatocrit >2 %), pancreatic enzymes replacement (yes/no), pubertal status-Tanner criteria (prepubertal: M1/G1, pubertal: M2/G2 to M4/G4, postpubertal: M5/G5), age at CF diagnosis (<2 and ≥2 years of age), Lung function, measured as a predicted forced expiratory volume in 1 s (FEV1). Main outcome measures Nutritional indicators: body mass index for age (BMI/A) and height for age (H/A) with z-score calculated with Anthro Plus software. Cut-off reference points: ≥ z-score −3 and < z-score −2 (thinness); z-score −2 and ≤ z-score-z +1 (normal weight); >z-score +1 (overweight or obesity), and z-score <−2 (low or very low H/A). The groups were compared by the Kruskal-Wallis test. Level of significance: p < 0.05.
Thirty adolescents. Median (min;max) age: 14.4 (10.1;19.8) years. BMI/A and H/A z-score, respectively: early diagnosis of CF (−0.8; −1.1) or late diagnosis of CF (−0.5;-0.8); with pancreatic insufficiency (−0.7; −0.8) or without pancreatic insufficiency (−0.8; −0.5) and prepubertal (−0.8; −0.7) pubertal (−0.2; −1.5) or postpubertal (−0.7; -0.5). No significant difference (p > 0.05) was observed. Patients with and without pancreatic insufficiency, presented H/A borderline z-score (p = 0.05). Association between H/A and FEV1 was borderline (p = 0.05).
Adolescents presented adequate nutritional status, although with slightly lower values than those of developed countries. FEV1 lower levels occurred more frequently in adolescents with low H/A.
- Cystic fibrosis
- Nutritional status
- Pancreatic insufficiency
Adolescence is a phase of life developing from 10 to 19 years which is characterized by an evolving period with marked body modifications and a high nutritional demand and culminating with biopsychosocial maturation . Rapid linear growth, the pubertal spurt and sexual maturation occur during this period and are evaluated according to the different stages of the Tanner classification (M2 to M4 in girls and G2 to G4 in boys) . This second decade of life is considered to be highly vulnerable since several factors can interfere with the full physical and emotional growth of adolescents, among them chronic diseases .
Cystic fibrosis (CF) is a lethal genetic disease of recessive autosomal inheritance commonly affecting Caucasian populations. CF is due to mutation of the Cystic Fibrosis Transmembrane Conductance Regulator gene which induces the production of thick and viscous secretions that obstruct the lungs, the pancreas and the bile duct. Among various therapeutic measures, this disease requires the use of pancreatic enzymes for patients with pancreatic insufficiency and a high-protein and high-calorie diet, which are associated with better pulmonary function and longer survival .
Over the last few years, due to advances in the early detection of the disease and to new treatments, the prognosis of CF patients has improved, with an increase in time and quality of life .
The objective of the present study was to determine the nutritional status of adolescents seen at a reference center and to associate it with the periods of puberty, with pancreatic sufficiency, with pulmonary function and with the age range of CF diagnosis.
An observational, cross-sectional, retrospective and analytical study was conducted using the data of medical records of the Reference Center of Cystic Fibrosis of the University Hospital. The city of Ribeirão Preto has a Human Development Index of 0.800 and serves a population estimated at more than two million inhabitants residing in the northeastern region of the state of São Paulo. All adolescents treated in this service since its creation, with a diagnosis of CF based on clinical manifestations and two sweat chloride tests >60 mEq/L, and who had attended at least one medical visit in 2010 were included in the study. There were 136 patients attended in 2010 and 30 was adolescents. The sample size was 28 (IC 90 %), according with statcalc Epi-info seven, considering all patients attended and prevalence of 25 % of undernutrition in infants and adolescents with cystic fibrosis. However, we chose to work with convenience sample. As there were no losses, all 30 teenagers participated in the study. The variables studied were age (years), sex (M/F), reported color (white non-white), weight (g), height (cm), pancreatic sufficiency defined according to steatocrit >2 % and generating two subgroups, i.e., patients with pancreatic sufficiency (PS) and patients with pancreatic insufficiency (PI), use of pancreatic enzymes (yes/no), use of nutritional supplement (yes/no), chronic colonization to pseudomonas aeruginosa (yes/no), cystic fibrosis related diabetes - CFRD (yes/no), cystic fibrosis related hepatic disease - CFRHD. Lung function (forced expiratory volume in 1 s, measured as a percent of predicted normal values - FEV1). Biohumoral indirect parameters of patient’s nutritional state levels: haemoglobin g/dL (Hb), total protein g/dL (TP) and albumin g/dL. According to the Tanner criteria, pubertal status was categorized as prepubertal (M1/G1), pubertal (M2/G2 to M4/G4) and postpubertal (M5/G5) and the age range at CF diagnosis was defined as <2 and ≥2 years of age.
Anthropometric data such as weight and height were used to determine the nutritional status. The anthropometric indicators adopted were body mass index for age (BMI/A) and height for age (H/A) according to chronological age and sex. BMI was calculated as weight (kg) divided by height (m) squared. The z-score of the nutritional indicators was calculated with the Anthro Plus software using the cut-off reference points established by the WHO : ≥ z-score −3 and < z-score −2 (thinness); z-score −2 and ≤ z-score-z +1 (normal weight); > z-score +1 (overweight or obesity), and z-score <−2 (low or very low H/A). The groups were compared by univariate analysis using the Kruskal-Wallis test of the EPI INFO 7 software, with the level of significance set at p ≤ 0.05.
The study was approved by the Research Ethics Committee of the University Hospital Ribeirão Preto.
Characteristics of the 30 adolescents evaluated at the Reference Center of Cystic Fibrosis of HC - USP, 2010
Presence of pancreatic insufficiency
Use of pancreatic enzymes
Use of enzymes according to pancreatic sufficiency
With pancreatic insufficiency
With pancreatic sufficiency
Age at CF diagnosis
Age at diagnosis of pancreatic insufficiency
Pancreatic insufficiency in patients diagnosed before 2 years of age
FEV1 medians (min; max)was 78.5 (41.0; 111) and 82.5 (45.0;98.0) considering that diagnosed before and after 2 years old, respectively (p > 0.05); 77.0 (45.0;102.0) and 85.0 (41.0;111.0) to the PI and PS, respectively (p > 0.05) and yet 82.0 (51.0;97.0); 86.0 (66.0;92.0) and 71.5 (41.0;111.0) to prepubertal, pubertal and postpubertal subgroups, respectively (p > 0.05).
FEV1 medians were 51.0 (51.0;51.0); 82.0 (41.0;111.0) e 88.0 (88.0;88.0) to thinness, normal weight and overweight or obesity (p > 0.05) and were 68.0 (45.0;88.0) and 84.0 (41.0;111.0) to low height and normal height, respectively (p = 0.05).
The FCDR was present in 13.3 % (4/30) and the FCDR in 26.7 % (8/30) of the adolescents.
BMI/A medians (min; max) was −0.64 (−3.7;+2.6) and −0.8 (−1.2;+0,7) to that without or with FCRD (p > 0.05) and BMI/A medians (min; max) was −0.75 (−3,7;+2.6) and −0.41 (−1.0;+0.74) to adolescents without or with FCDHR (p > 0.05), respectively. H/A means (SD) was −0.83 (−3.8;1.2) and −1.38 (−2.2;-0.18) to adolescents without or with FCDR (p > 0.05) and H/A means was −0.75 (−3.7;+1.2) and −1.0 (−2.8;+0.9) to adolescents without or with FCDHR, respectively (p > 0.05).
Considering all 30 adolescents, 73.3 % (22/30) were taking nutritional supplement. BMI/A medians (min; max), were 0.2 (−1.2;+2.6) and −0.72 (−3.7;+0.7) and HAZ means were −0.4 (−3.3;1.2) and −1.0 (−3.7;+0,9) to that adolescents without or taking nutritional supplement, respectively (p > 0.05).
Among prepubertal, pubertal and postpubertal subgroups it were observed the medians (min;max) of Hb 13.4 (11.9;14.7), 13.7 (11.7;15.6) and 13.6 (10.3;16.5); TP 7.1 (6.7;8.0), 6.8 (6.3;7.9) and 7.5 (6.1;7.8) and albumin 4.3 (3.9;4.7), 4.3 (3.6;4.6) and 4.1 (3.3;4.6); respectively (p > 0,05).
Distribution of the variables according to age, BMI/A and H/A of the 30 adolescents evaluated at the Reference Center of Cystic Fibrosis of HC - FMRP - USP, 2010
Age at Cystic Fibrosis diagnosis
BMI/A a (z score)
Age at Cystic Fibrosis diagnosis
H/A b (z score)
Age at Cystic Fibrosis diagnosis
Before 2 years
After 2 years
The results observed in this study allow the discussion, then, of some aspects. BMI/A has been recently adopted as a parameter for the surveillance of nutritional status in CF patients older than 2 years, and values above the 50th percentile are known to correlate with better pulmonary function . A BMI/A < 15th percentile (p15) has been indicated as the ideal cut-off point for the definition of malnutrition in CF patients and the Cystic Fibrosis Foundation has recommended the BMI/A > 50th percentile as the goal to be reached for the nutritional status of these individuals . Although without the rigor of the parameters adopted for consensus , in the present study the adolescents showed a BMI/A z-score of −0.6 (−3.7; 2.6) which corresponds to adequate nutritional status according to the parameters of the World Health Organization . A study conducted in Germany on CF patients aged 4 to 18 years detected a BMI/A z-score of −0.1 .
The BMI/A z-score was similar for the present adolescents with and without pancreatic insufficiency (−0.7 and −0.8; respectively), whose nutritional status, therefore, was of adequate level according to WHO standards. These findings were close to those detected in an Italian study on patients younger than 18 years whose BMI/A z-scores were 0.2 and −0.3 for PS and PI, respectively . It should also be pointed out that all the present adolescents with PI took pancreatic enzymes, which are extremely important for the maintenance of nutritional status in such patients .
It is known that, the sooner the diagnosis of CF is established, the more serious the symptoms and the shorter the survival . However, in the present study, BMI/A z-scores did not differ significantly between an early or late diagnosis of CF, being −0.8 and −0.5, respectively. Although the present patients were not routinely genotyped, the results obtained agree with those published by investigators from developed countries, who reported that some patients with the classical manifestations of CF experience a mild disease course and that, in addition, an important proportion of patents with the severe and common delta F 508 mutation survive to 40 years of age with good maintenance of weight and pulmonary function .
Also concerning the nutritional status, the present group of prepubertal adolescents obtained the lowest BMI/A z-score (−0.8). The pubertal and postpubertal adolescents had z-scores of −0.2 and −0.7, respectively, all of them being considered nutritionally adequate. Because in this phase of life complications such as hepatic diseases, diabetes related to CF or even worsening of pulmonary function may further aggravate the nutritional status, more complications may have occurred in the prepubertal group, since these factors were not controlled due to the limited sample size [15, 16].
However, the better BMI/A z-scores detected in the pubertal and postpubertal groups may have been due not only to a satisfactory weight gain, but also to the lack of a height gain adequate for age, since this indicator underestimates overweight and underdiagnoses short stature in children and adolescents [17–19], a fact that underscores the importance of evaluating the H/A indicator.
The H/A ratio expresses linear growth and is considered to be the nutritional indicator that best points out the cumulative effect of adverse situations and the most sensitive one for the determination of quality of life in a population . A low H/A (<5th percentile) has been recognized as a prognostic indicator of survival for CF patients . Among CF individuals, adolescence is a period of high risk for delayed pubertal growth spurt  and impaired final height [23, 24], which appears to be mitigated by a better nutritional status at 7 years of age .
The H/A z-score of −0.8 (−3.7; 1.2) detected in the present patients is within adequate levels according to WHO standards, without corresponding to a low H/A, which characterizes worse survival among CF patients. Similar results were obtained by Groeneweg et al. in Germany .
No significant difference in H/A z-score was observed between patients with and without PI, although the borderline value of p = 0.05 may indicate impaired height in PI patients compared to PS patients. Indeed, the nutritional status of individuals with PS could be expected to be better than that of individuals with PI, a result also observed in a study conducted in Italy, in which significant differences in z-score (p < 0.03) were detected between PS and PI subjects .
Regarding the different stages of adolescence, the H/A z-scores did not differ significantly (p > 0.05) between prepubertal (−0.7), pubertal (−1.5) and postpubertal (−0.5) adolescents. Similarly H/A z-scores did not differ significantly (p > 0.05) between the groups diagnosed with CF before (−1.0) or after two years of age (−1.3). The different stages of adolescence and the early or late diagnosis of CF were not sufficient to differentiate the nutritional status of the various adolescent subgroups studied, with adequate H/A levels being observed in all of them according to the WHO.
The pulmonary function between BMI/A subgroups was not significant (p > 0.05), although 51.0 % adolescents with thinness presented lower FEV1 median. In turn, lower FEV1 means observed in 65.5 % adolescents with low height revealed that pulmonary function between H/A subgroups was significant (p = 0.05). Other researchers observed strong association between FEV1 and weight-for-height (ref. ).
The retrospective collection of anthropometric data is one of the limitations of the present study, although weight and height were measured by personnel trained by specialists. Adequate means of Hb, PT and albumin, corroborates the means of the anthropometric nutritional indicators observed in this study. The absence of genotyping and the non-exclusion of patients with major complications such as liver disease and diabetes related to CF are also limitations, which, however, did not overestimate the values observed.
Although the need for a more rigorous nutritional assessment of individuals with CF is recognized, the adolescents studied here showed adequate nutritional status related to BMI/A and H/A, although with slightly lower values than those of developed countries according to WHO standards.
Parameters regarding an early or late diagnosis of CF and the pubertal stage of these adolescents did not reveal differences in nutritional status when BMI/A or H/A were considered.
BMI/A values did not differ in terms of status of pancreatic sufficiency, whereas H/A values were borderline for the determination of height impairment in adolescents with PI compared to adolescents without PI. FEV1 lower levels occurred more frequently in adolescents with low H/A.
- World Health Organization. Health for the World’s Adolescents. A second chance in the second decade, 2014. http://www.who.int/maternal_child_adolescent/topics/adolescence/second-decade/en/ (accessed 01 May 2014).
- Tanner JM, Whitehouse RH. Clinical longitudinal standards for height, weight, height velocity, weight velocity, and stages of puberty. Arch Dis Child. 1976;51:170–80.PubMed CentralPubMedView ArticleGoogle Scholar
- Rosen DS. Pubertal growth and sexual maturation for adolescents with chronic illness or disability. Pediatrician. 1991;18:105–20.PubMedGoogle Scholar
- Peters C. Cystic fibrosis: a review of pathophysiology and current treatment recommendations. S D Med. 2014;4:158–51.Google Scholar
- Boyle MP, De Boeck K. A new era in the treatment of cystic fibrosis: correction of the underlying CFTR defect. Lancet Respir Med. 2013;1:158–63.PubMedView ArticleGoogle Scholar
- World Health Organization. The WHO child growth standards, 2006. http://www.who.int/childgrowth/en/. Accessed 17 jun 2015.
- Stallings VA, Stark LJ, Robinson KA, Feranchak AP, Quinton H. Clinical Practice Guidelines on Growth and Nutrition Subcommittee; Ad Hoc Working Group. Evidence-based practice recommendations for nutrition-related management of children and adults with cystic fibrosis and pancreatic insufficiency: results of a systematic review. J Am Diet Assoc. 2008;108(5):832–9.PubMedView ArticleGoogle Scholar
- Lai HC, Shoff SM. Classification of malnutrition in cystic fibrosis: implications for evaluating and benchmarking clinical practice performance. Am J Clin Nutr. 2008;88:161–6.PubMedGoogle Scholar
- Borowitz D, Baker RD, Stallings V. Consensus report on nutrition for pediatric patients with cystic fibrosis. J Pediatr Gastroenterol Nutr. 2002;35:246–59.PubMedView ArticleGoogle Scholar
- Groeneweg M, Tan S, Boot AM, de Jongste JC, Bouquet J, Sinaasappel M. Assessment of nutritional status in children with cystic fibrosis: conventional anthropometry and bioelectrical impedance analysis. A cross-sectional study in Dutch patients. J Cyst Fibros. 2002;1:276–80.PubMedView ArticleGoogle Scholar
- Lucidi V, Alghisi F, Raia V, Russo B, Valmarana R, et al. Growth Assessment of Paediatric Patients With CF Comparing Different Auxologic Indicators: A Multicentre Italian Study. J Pediatr Gastroenterol Nutr. 2009;49:335–42.PubMedView ArticleGoogle Scholar
- Anthony H, Collins CE, Davidson G, Mews C, Robinson P, Shepherd R, et al. Pancreatic enzyme replacement therapy in cystic fibrosis: Australian guidelines. J Paediatr Child Health. 1999;35:125–9.PubMedView ArticleGoogle Scholar
- Kulich M, Rosenfeld M, Goss CH, Wilmott R. Improved survival among young patients with cystic fibrosis. J Pediatr. 2003;142:631–6.PubMedView ArticleGoogle Scholar
- Simmonds NJ, Cullinan P, Hodson ME. Growing old with cystic fibrosis: the characteristics of long-term survivors of cystic fibrosis. Respir Med. 2008;103:629–35.PubMedView ArticleGoogle Scholar
- Rosenecker J, Höfler R, Steinkamp G, Eichler I, Smaczny C, Ballmann M, et al. Diabetes mellitus in patients with cystic fibrosis: the impact of diabetes mellitus on pulmonary function and clinical outcome. Eur J Med Res. 2001;6(8):345–50.PubMedGoogle Scholar
- Rowland M, Gallagher C, Gallagher CG, Laoide RO, Canny G, Broderick AM, et al. Outcome in patients with cystic fibrosis liver disease. J Cyst Fibros. 2014;S1569–1993(14):00119–2.Google Scholar
- Lai HJ. Classification of nutritional status in cystic fibrosis. Curr Opin Pulm Med. 2006;12:422–7.PubMedView ArticleGoogle Scholar
- Zhang Z, Lindstrom MJ, Lai HJ. Pubertal height velocity and associations with prepubertal and adult heights in Cystic Fibrosis. J Pediatr. 2013;163:376–82.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang Z, Lai HJ. Comparison of the use of body mass index percentiles and percentage of ideal body weight to screen for malnutrition in children with cystic fibrosis. Am J Clin Nutr. 2004;80:982–91.PubMedGoogle Scholar
- Brasil. Ministério da Saúde. Protocolos do Sistema de Vigilância Alimentar e Nutricional, 2008. http://188.8.131.52/dab/docs/portaldab/publicacoes/orientacoes_coleta_analise_dados_antropometricos.pdf. Accessed 17 jun 2015.
- Beker LT, Russek-Cohen E, Fink RJ. Stature as a prognostic factor in cystic fibrosis survival. J Am Diet Assoc. 2001;101:438–42.PubMedView ArticleGoogle Scholar
- Landon C, Rosenfeld RG. Short stature and puberal delay in cystic fibrosis. Pediatrician. 1987;14:253–60.PubMedGoogle Scholar
- Aswani N, Taylor CJ, McGaw J, Pickering M, Rigby AS. Pubertal growth and development in cystic fibrosis: a retrospective review. Acta Paediatr. 2003;92:1029–32.PubMedView ArticleGoogle Scholar
- Yen EH, Quinton H, Borowitz D. Better nutritional status in early childhood is associated with improved clinical outcomes and survival in patients with cystic fibrosis. J Pediatr. 2013;162(3):530–5.PubMedView ArticleGoogle Scholar
- Martínez-Costa C, Escribano A, Núñez Gómez F, García-Maset L, Luján J, Martínez-Rodriguez L. Nutritional intervention in children and adolescents with cystic fibrosis. Relationship with pulmonary function. Nutr Hosp. 2005;20(3):182–8.PubMedGoogle Scholar
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