- Open Access
Glutathione S transferase theta1 and mu1 gene polymorphisms and phenotypic expression of asthma in Egyptian children: a case–control study
© El Rifai et al.; licensee BioMed Central Ltd. 2014
- Received: 6 September 2013
- Accepted: 19 February 2014
- Published: 21 February 2014
Asthma is the result of a complex interaction between environmental factors and genetic variants that confer susceptibility. The glutathione S-transferases (GSTT1 and GSTM1) are phase II enzymes thought to protect the airways from oxidative stress. Few and contradictory data are available on the association between asthma development and GSTT1 and GSTM1 polymorphisms in different ethnic groups. The current study aimed to investigate whether these polymorphisms are associated with asthma development in the Egyptian population.
The cross-sectional study was performed on 94 asthmatic children 6 -12 yrs and 90 matched healthy controls. Candidates were subjected to clinical evaluation and measurement of absolute blood eosinophilic count, total serum IgE, and GSTT1 and GSTM1 genotype by multiplex PCR technique.
The results for GSTT1 null genotype were 87.2% and 97.2% for asthmatic children and controls respectively and showed to be significantly more in controls (P =0.007, OR:0.683, CI: 0.034 -0.715). The results for GSTM1 null genotype were 50% and 61.1% for asthmatic children and controls respectively and showed to be nonsignificant (p = 0.130, OR: 1.000, CI: 0.54- 1.86). Also, no association was detected between GSTT1 and GSTM1 polymorphisms and atopic conditions or asthma severity.
The significant detection o f GSTT1 null genotype more in controls than in asthmatics with no association with other atopic manifestations or asthma severity and the lack of association detected between GSTM1 polymorphism in relation to asthma, atopy or asthma severity confirm the uncertain role of those genes in the development of asthma.
- Glutathione S-transferase
Asthma is a disorder of the airways characterized by several symptoms such as airflow obstruction, airway inflammation, and hyper responsiveness . The study of genetic factors involved in complex pathologies such as asthma is arduous, not only because of human genetic variability, or incomplete penetrance, but also because, in complex disease studies, the importance and strength of gene to gene and gene to environment interactions need to be considered . The prevalence of candidate gene polymorphisms for asthma varies considerably worldwide, and accordingly, ethnicity should be considered as a factor that might act on and influence asthma development. Previous data based on intra- and inter-population frequency differences suggest that the association between a given genetic polymorphism and asthma cannot be extrapolated from one ethnic group to another .
Phase II detoxification enzymes, particularly classes of GSTs, play an important role in inflammatory responses triggered by xenobiotic or reactive oxygen compounds . The GSTM1 and GSTT1 are two important phase II enzymes that protect the airways from oxidative stress . They utilize as substrates a wide variety of products of oxidative stress . The inability of GST variant enzymes to detoxify reactive oxygen species contributes to the activation of the inflammatory process, bronchoconstriction, and the exacerbation of asthma symptoms . In particular, GSTM1 and GSTT1 null polymorphisms may influence the pathogenesis of respiratory diseases. Numerous studies have documented associations between genes implicated in the oxidative stress response and respiratory phenotypes, but data suggest that they may not be consistent across ethnic groups owing to differences in intra- and inter-ethnic allele frequencies .
The aim of the current study was to detect the presence of an association between GSTM1 and GSTT1 polymorphisms and asthma, atopy or asthma severity.
The present cross-sectional case–control study is conducted on a group of Egyptian asthmatic children (n: 94) and their age and sex matched healthy controls (n: 90) from September 2012 to June 2013. Patients were recruited from the allergy clinic of Cairo university specialized pediatric hospital where they were following up after being diagnosed according to GINA guidelines criteria of asthma classification . All patients were subjected to a questionnaire containing a detailed history and clinical examination with emphasis on age, sex, family history, presence of atopic manifestations and asthma severity classification according to GINA Guidelines . The following investigations were performed for all patients and controls.
Total immunoglobulin E (IgE) and Prick test assays
Atopy was defined by a positive history of atopic manifestations, positive skin prick test (wheal diameter ≥3 mm) to at least one of the following aeroallergens (Dermatophagoides Farinae, hay Dust, Dermato-pteronyssinus, Alternaria Tenuis, Moulds II, Candida Albicans, Cat epithelia, Hen’s egg, Dog epithelia, Grasses/cereals, Cow’s milk in the presence of positive histamine control and negative physiological saline control using reagents obtained from Allergopharma D21462 Reinbek, Germany) and by the quantitative determination of human total IgE in serum using the DiaMed Eurogen IgE quantitative technique which is a monoclonal antibody based enzyme immunoassay (Positive values were taken to be ≥200 IU/ml). Among the 94 asthmatic children there were 67 atopic and 27 nonatopic children.
Pulmonary function tests assay
Spirometric measurements using a Jaeger Master Screen Spirometry system (Jaeger Co) were done and included forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and forced expiratory flow between 25% and 75% expired volume (FEF25-75). Short-acting bronchodilators were stopped at least 8 h before the test. All pulmonary function data were collected at a single visit. A minimum of 3 results within 10% of each other were recorded, and the result with the highest FEV1 was analyzed. The participants were not suffering from asthma exacerbations or other acute illnesses at the time of the measurement of pulmonary function. The lung function test results were expressed as a percentage of that predicted.
The personal, family, medical history and clinical presentation of controls were free of any atopic or allergic diseases with negative skin prick tests, normal total IgE values, normal lung function tests.
Genotyping of GSTT1 and GSTM1
100 ng of DNA were amplified in a-50 ul multiplex reaction mixture containing 0.90 pmol of each of the following GSTT1 primers (GSTT1-Forward: GAACTCCCTGAAAAGCTAAAGC and GSTT1-Reverse: GTTGGGCTCAAATATACGGTGG) and GSTM1 primers (GSTM1-Forward: TTCCTCACTGGTCCTCACATCTC and GSTM1-Reverse: TCACCGGATCATGGCCAGCA). As an internal control, the beta-globulin gene was also amplified using the following amplification sequence (Forward primer: GCCCTCTGCTAACAAGTCCTAC and Reverse primer: GCCCTAAAAAGAAAATCGCCAATC) . The amplification reaction consisted of 0.9 pmol of each primer added to 12.5 u PCR master mix which contains 3.5 mM MgCl2, 200 uM dNTPs, 5 ul 10X PCR buffer, and 2U TaqDNA polymerase (Fermentas).
The PCR protocol included: initial melting temperature of 94°C (5 minutes), amplification by 35 cycles of 20 seconds at 94°C, 20 seconds at 64°C, and 30 second at 72°C) then final extension at 72°C for 7 minutes. Analysis of PCR products on agarose gels where a fragment of 215 pb indicated the presence of GSTM1, a fragment of 480 pb indicated the presence of GSTT1 and a fragment of 280 pb indicated the positive internal control B globulin. The subjects were classified as either (+), when at least one specimen of the gene was detected, or (-) when they showed a null genotype.
The aim and nature of the study was explained for each candidate and/or parent before inclusion. An informed written consent was obtained from parents/surrogates before enrollment. Children old enough were asked for consent. Cairo University Hospital Research Ethical Committee approved the work and it conforms to the provisions of the Declaration of Helsinki in 1995 (as revised in Seoul 2008).
Data were statistically described in terms of mean ± standard deviation (± SD), median and range, or frequencies (number of cases) and percentages when appropriate. Comparison of numerical variables between the study groups was done using Student’s t test for independent samples when comparing 2 groups of normally distributed data and Mann Whitney test when comparing 2 groups of non-normal data. Kruskal Wallis test with posthoc multiple 2-group comparisons was used to compare numerical data between more than 2 groups. For comparing categorical data, Chi square (χ2) test was performed. Exact test was used instead when the expected frequency is less than 5. Odds ratio with its 95% CI was used to present the relation between haplotypes in cases and controls. Haldane modification was used when the occurrence of any haplotypes was zero. p values less than 0.05 was considered statistically significant. All statistical calculations were done using computer programs SPSS (Statistical Package for the Social Science; SPSS Inc., Chicago, IL, USA) version 15 for Microsoft Windows.
Characteristics and genotype distributions in asthmatics and controlsª
Age (Mean ± SD), years
7.65 ± 1.916
8.27 ± 1.785
97 ± 6.2
96 ± 8.2
Passive smoking exposure
Genotype distributions in atopic asthmatics and non-atopic asthmaticsª
n = 67 (%)
n = 27 (%)
Genotype distributions in asthmatics stratified by disease severityª
n = 24 (%)
n =36 (%)
N = 34 (%)
It was recently recognized that GSTs play an active role in oxidative defenses and members of this superfamily may be determinants of respiratory health . The presence of the GSTT1 null polymorphism was 87.2% and 97.2% in asthmatics and controls respectively and the results showed to be significantly higher in controls (P = 0.007, OR:0.683, CI: 0.034 -0.715). The results for GSTM1 were 50% and 61.1% respectively and showed to be nonsignificant (p = 0.130, OR: 1.000, CI: 0.54- 1.86). A systematic review and meta-analysis for the effects of GST genes on asthma demonstrated that a large Avon Longitudinal Study of Parents and Children found a protective effect on asthma of the GSTT1 null allele in mothers (OR: 0.71; 95% CI: 0.57–0.90 and 0.84; 0.63–1.12, for heterozygotes and null homozygotes, respectively, compared with wild-type homozygotes) and children (0.91; 0.76–1.11 and 0.89; 0.70–1.13) . No association of the disease with GSTT1 null genotype was noted by several studies in different populations [2, 12–20]. However, in contrary to our findings an increased risk was seen in individuals with this genotype in some studies [3, 21–24]. Similarly, a study performed on Egyptian population in Zagazig -located in northern Egypt- showed that asthmatic children had a significant lower prevalence of GSTT1 null genotype than the control group (P = 0.003). However, a higher prevalence of the GSTM1 null genotype was observed in the asthmatic group . As our study was performed in Cairo- the capital of Egypt located in the centre- the enrolled patients were referred from upper Egypt that’s why a difference in GSTM1 null genotype frequencies among asthmatics and controls in the two studies.
In agreement with the current study results, no association of the disease with GSTM1 null genotype was noted by several studies in different populations [2, 12–16, 18]. However, an increased risk was seen in individuals with this genotype in some reports [3, 17, 19–24]. The fact that asthma pathogenesis is a result of interactions between multiple genetic and environmental factors highlight that exposure to environmental chemical agents may explain the differences encountered.
A recent study done in Italy for asthmatic adults- which revealed no association for both null genotypes with the disease- stated that the differences in the prevalence of asthma in different ethnic groups reflect and highlight genetic variances with a significant coverage of environmental conditions. The study declared that rapid change in asthma prevalence is not linked to genetic changes in populations because these mechanisms are too slow to explain this scenario and the effect of environmental exposures and interactions between genetic factors and environmental conditions are still a matter of debate .
The current study revealed that the frequencies of GSTT1 and GSTM1 null genotypes in controls were 97.8% and 60.1% respectively. The frequencies previously reported in the Egyptian population (15% and 44% respectively among 34 subjects)  and in another study a higher frequencies was reported (25.50% and 55.50% respectively) . According to our results, the frequency of the GSTM1 null genotype (60.1%) was slightly higher than the previous Egyptian studies, comparable to Caucasians (50.4% -58%), Europeans (39.00–62.00%) and White Americans (35.00–62.00%). However, the frequency of the GSTT1 null genotype (97.8%) was extremely higher than the range of the previous Egyptian studies, Europeans (10.00–21.00%), Africans (15.00–26.00%) and Caucasian- Americans (10.00–36.00%).
Differences in gene frequencies among various ethnic groups, may explain the differences encountered. A previous study that attempted to investigate the prevalence of important allelic variants of several genes including GST gene in the Egyptian population denoted that Egypt is unique geographically, as it is located centrally to the three continents of Africa, Europe and Asia, so its population is highly affected by the rapid pace of intercontinental transportation and large-scale immigration and that throughout history, the Greeks, Romans, Arabs, Turks, French and British have all ruled Egypt and mixed with its people, so that modern Egypt now is an amalgam of all these legacies. So, there is a considerable genetic admixture in the Egyptian population . This genetic admixture explains more the differences encountered between the results of the current study done in Cairo- central Egypt- and that performed in Zagazig- northern Egypt.
The present study compared the GSTT1and GSTM1 null genotypes among asthmatic patients with the levels of asthma severity whether mild (87.5% and 50% respectively), moderate (80.6% and 52.8% respectively) or severe persistent (94.1% and 47.1% respectively) and it revealed no statistical significance (P value: 0.236 and 0.892 respectively). These findings were supported by several studies [12, 14, 15, 29, 30].
Regarding the genetic polymorphisms of GSTT1 and GSTM1 among atopic asthmatic patients and non-atopic asthmatics, no significant statistical difference in GSTT1 and GSTM1 polymorphisms was noted between the two groups (P value: 0.706 and 0.820 respectively). In agreement with the current study, a study done on Egyptian population in zagazig announced that there is no significant association found between atopy and GSTT1 polymorphism. However, they found that the GSTM1 null genotype was significantly higher in atopic asthmatic cases than in nonatopic asthmatic subjects (P = 0.01) .
Comparison of our results with other studies indicates that GSTT1 and GSTM1 null genotypes were not universally associated with the asthma phenotypes. The genetic basis of asthma may differ between different ethnic groups. Future studies of large size should focus on interactions of GST genes with environmental oxidative exposures and with other genes involved in antioxidant pathways. Quality of study conduct and reporting need to be improved to increase credibility of the evidence accumulating over time.
The study was self-funded by authors.
- Lemanske RF, Busse WW: Asthma: clinical expression and molecular mechanisms. J Allergy Clin Immunol. 2010, 125: 95-102. 10.1016/j.jaci.2009.10.047.View ArticleGoogle Scholar
- Holla LI, Stejskalova A, Vasku A: Polymorphisms of the GSTM1 and GSTT1 genes in patients with allergic diseases in the Czech population. Allergy. 2006, 61 (2): 265-267. 10.1111/j.1398-9995.2006.01000.x.View ArticlePubMedGoogle Scholar
- Hanene C, Jihene L, Jamel A, Kamel H, Agnès H: Association of GST genes polymorphisms with asthma in Tunisian children. Mediators Inflamm. 2007, 37: 1150-1157.Google Scholar
- Goodrich GG, Goodman PH, Budhecha SK, Pritsos CA: Functional polymorphism of detoxifi cation gene NQO1 predicts intensity of empirical treatment of childhood asthma. Mutat Res. 2009, 674: 55-61. 10.1016/j.mrgentox.2008.10.009.View ArticlePubMedGoogle Scholar
- Hayes JD, Flanagan JU, Jowsey IR: Glutathione transferases. Annu Rev Pharmacol Toxicol. 2005, 45: 51-88. 10.1146/annurev.pharmtox.45.120403.095857.View ArticlePubMedGoogle Scholar
- Hayes JD, Strange RC: Potential contribution of the glutathione S-transferase supergene family to resistance to oxidative stress. Free Radic Res. 1995, 22: 193-197. 10.3109/10715769509147539.View ArticlePubMedGoogle Scholar
- Reddy P, Naidoo RN, Robins TG, Mentz G, London SJ, Li H, Naidoo R: GSTM1, GSTP1, and NQO1 polymorphisms and susceptibility to atopy and airway hyperresponsiveness among South African schoolchildren. Lung. 2010, 188: 409-414. 10.1007/s00408-010-9246-3.PubMed CentralView ArticlePubMedGoogle Scholar
- Global Strategy for the Diagnosis and Management of Asthma, Global Initiative for Asthma (GINA), revised 2010. http://www.ginasthma.com.
- Sheikhha MH, Kalantar M, Tobal K, John A, Yin L: Glutathione S-transferase null genotype in acute myeloid leukemia. IJI. 2005, 2: 141-151.Google Scholar
- Gilliland FD, Gauderman WJ, Vora H, Rappaport E, Dubeau L: Effects of glutathione-S-transferase M1, T1, and P1 on childhood lung function growth. AM J Respir Crit Care Med. 2002, 166 (5): 710-716. 10.1164/rccm.2112065.View ArticlePubMedGoogle Scholar
- Minelli C, Granell R, Newson R, Rose-Zerilli M, Torrent M, Ring S, Holloway JW, Shaheen SO, Henderson JA: Glutathione-S-transferase genes and asthma phenotypes: a Human Genome Epidemiology (HuGE) systematic review and meta-analysis including unpublished data. Int J Epidemiol. 2010, 39: 539-562. 10.1093/ije/dyp337.PubMed CentralView ArticlePubMedGoogle Scholar
- Freĭdin MB, Bragina EI, Ogorodova LM, Puzyrev VP: Polymorphism of the theta1 and mu1 glutathione S-transferase genes (GSTT1, GSTM1) in patients with atopic bronchial asthma from the West Siberian region. Mol Biol. 2002, 36 (4): 630-634.Google Scholar
- Ercan H, Birben E, Dizdar EA, Keskin O, Karaaslan C, Soyer OU, Dut R, Sackesen C, Besler T, Kalaysi O: Oxidative stress and genetic and epidemiologic determinants of oxidants injury in childhood asthma. J Allergy Clin Immunol. 2006, 118: 1097-1104. 10.1016/j.jaci.2006.08.012.View ArticlePubMedGoogle Scholar
- Mak JC, Ho SP, Leung HC, Cheung AH, Law BK, So LK, Chan JW, Chao CH, Lam WK, Ip MS, Chan-Yeung M: Relationship between glutathione s-transferase gene polymorphisms and enzyme activity in Hong Kong Chinese asthmatics. Clin Exp Allergy. 2007, 37 (8): 1150-1157. 10.1111/j.1365-2222.2007.02704.x.View ArticlePubMedGoogle Scholar
- Salam MT, Lin PC, Avol EL, Gauderman WJ, Gilliland FD: Microsomal epoxide hydrolasem glutathione Stransferase P1, traffic and childhood asthma. Thorax. 2007, 62 (12): 1050-1057. 10.1136/thx.2007.080127.PubMed CentralView ArticlePubMedGoogle Scholar
- Imboden M, Rochat T, Brutsche MH, Schindler C, Downs SH, Gerbase MW, Burger W, Probst- Hensch NM, SAPALDIA Tea: Glutathione S-transferase genotype increases risk progression from bronchial hyperresponsiveness to asthma in adults. Thorax. 2008, 63 (4): 322-328. 10.1136/thx.2007.085555.View ArticlePubMedGoogle Scholar
- Islam T, Berhane K, McConnell R, Gauderman WJ, Avol E, Peters JM, Gilliland FD: Glutathione-S-transferase (GST) P1, GSTM1, exercise, ozone and asthma incidence in school children. Thorax. 2009, 64 (3): 197-202. 10.1136/thx.2008.099366.PubMed CentralView ArticlePubMedGoogle Scholar
- Castro-Giner F, Künzli N, Jacquemin B, Forsberg B, de Cid R, Sunyer J, Deborah J, David B, Danielle V, Dan N, González JR, Stefano G, Christer J, Josep-Maria A, Matthias W, Joachim H, Xavier E, Manolis K: Traffic-related air pollution, oxidative stress genes, and asthma (ECHRS). Environ Health Perspect. 2009, 117: 1919-1924. 10.1289/ehp.0900589.PubMed CentralView ArticlePubMedGoogle Scholar
- Rogers AJ, Brasch-Andersen C, Ionita-Laza I, Murphy A, Sharma S, Klanderman BJ, Raby BA: The interaction of glutathione S-transferase M1-null variants with tobacco smoke exposure and the development of childhood asthma. Clin Exp Allergy. 2009, 39: 1721-1729. 10.1111/j.1365-2222.2009.03372.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Lima CS, Néri IA, Lourenço GJ, Faria IC, Ribeiro JD, Bertuzzo CS: Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genetic polymorphisms and atopic asthma in children from Southeastern Brazil. Genet Mol Biol. 2010, 33: 438-441. 10.1590/S1415-47572010000300007.PubMed CentralView ArticlePubMedGoogle Scholar
- Ivaschenko TE, Sideleva OG, Baranov VS: Glutathione-S-transferase μ and theta gene polymorphisms as new risk factors of atopic bronchial asthma. J Mol Medic. 2002, 80 (1): 39-43. 10.1007/s001090100274.View ArticleGoogle Scholar
- Brasch-Andersen C, Christiansen L, Tan Q, Haagerup A, Vestbo J, Kruse TA: Possible gene dosage effect of glutathione-S-transferases on atopic asthma: using real-time PCR for quantification of GSTM1 and GSTT1 gene copy numbers. Hum Mutat. 2004, 24 (3): 208-214. 10.1002/humu.20074.View ArticlePubMedGoogle Scholar
- Saadat M, Saadat I, Saboori Z, Emad A: Combination of CC16, and GSTT1 genetic polymorphism is associated with asthma. J Allergy Clin Immunol. 2006, 113: 996-998.View ArticleGoogle Scholar
- Tamer L, MC a g, Ates NA, Yildirim H, Ercan B, Saritas E, Unlu A, Atik U: Glutathione-S-transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma. Respirology. 2004, 9 (4): 493-498. 10.1111/j.1440-1843.2004.00657.x.View ArticlePubMedGoogle Scholar
- Karam RA, Pasha HF, El-Shal AS, Rahman HM, Gad DM: Impact of glutathione-S-transferase gene polymorphisms on enzyme activity, lung function and bronchial asthma susceptibility in Egyptian children. Gene. 2012, 497: 314-319. 10.1016/j.gene.2012.01.059.View ArticlePubMedGoogle Scholar
- Piacentini S, Polimanti R, Moscatelli B, RE M, Manfellotto D, Fuciarelli M: Lack of association between GSTM1, GSTP1, and GSTT1 gene polymorphisms and asthma in adult patients from Rome, Central Italy. J Investig Allergol Clin Immunol. 2012, 22 (4): 252-256.PubMedGoogle Scholar
- Abdel-Rahman SZ, El-Zein RA, Anwar WA, Au WW: A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies. Cancer Lett. 1996, 107: 229-233. 10.1016/0304-3835(96)04832-X.View ArticlePubMedGoogle Scholar
- Hamdy SI, Hiratsuka M, Narahara K, Endo N, El Enani M, Moursi N: Genotype and allele frequencies of TPMT, NAT2, GST, SULT1A1 and MDR- 1 in the Egyptian population. J Clin Pharmacol. 2003, 55 (6): 560-569. 10.1046/j.1365-2125.2003.01786.x.View ArticleGoogle Scholar
- Sandford J, Chan HW, Wong GMK, Lai CKW, Chan-Yeung M: Candidate genetic polymorphisms for asthma in Chinese schoolchildren from Hong Kong. Intern J Tuberc Lung Dis. 2004, 8 (5): 519-527.Google Scholar
- Gattás GJ, Kato M, Soares-Vieira JA, Siraque MS, Kohler P, Gomes L, Rigo MA, Bydlowski SP: Ethnicity and glutathione S-transferase (GSTM1/GSTT1) polymorphisms in a Brazilian population. Braz J Med Biol Res. 2004, 37: 451-458.View ArticlePubMedGoogle Scholar
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